Nucleofill or Profhilo: Differences, Benefits, and Which One to Choose

Key Takeaways

• Nucleofill and Profhilo are both injectable skin boosters, but they work differently, Nucleofill is a polynucleotide-based biostimulator, while Profhilo is hyaluronic acid-based.
• Nucleofill promotes cellular regeneration, collagen production, and antioxidant repair, making it ideal for long-term rejuvenation and skin strengthening.
• Profhilo hydrates and plumps the skin quickly, offering visible results in elasticity and glow with fewer sessions.
• Patient selection depends on skin condition, desired outcome, and age, younger patients often benefit from Profhilo, while mature or compromised skin responds better to Nucleofill.
• Many professionals now use both treatments strategically, either in alternating sessions or combined protocols, for enhanced skin quality and structure.

Understanding the Basics: What Is Nucleofill vs. Profhilo?

Before diving into treatment planning, it’s essential to understand how these two boosters actually work.

What Is Nucleofill?

Nucleofill is a polynucleotide-based injectable designed to stimulate fibroblast activity, improve skin oxygenation, and promote deep tissue regeneration. It does not work like a filler or volumizer. Instead, it helps strengthen and rejuvenate the skin from within, making it especially beneficial for aging, sun-damaged, or stressed skin.

There are several formulations, including:

• Nucleofill Strong – for firming and restructuring mature or sagging skin
• Nucleofill Soft Eyes – a targeted solution for under-eye hollowness, puffiness, and fine lines

Want to learn more? Here’s a helpful guide: What is Nucleofill.

What Is Profhilo?

Profhilo is a high-concentration hyaluronic acid (HA) injectable that disperses through the skin, binding water molecules to hydrate and smooth tissue. It doesn’t act like a dermal filler but instead improves skin elasticity, hydration, and firmness by stimulating collagen and elastin.

Its unique feature is the Bio Aesthetic Points (BAP) injection technique, typically involving two sessions spaced 4 weeks apart.

Nucleofill or Profhilo: A Side-by-Side Comparison

Feature	Nucleofill	Profhilo
Main Ingredient	Polynucleotides (PDRN)	Hyaluronic Acid (HA)
Mode of Action	Biostimulation, collagen & elastin production, detox	Hydration, skin tightening, collagen stimulation
Best For	Regeneration, stressed/aged skin, inflammation	Hydration, fine lines, skin laxity
Treatment Areas	Face, neck, under eyes (Soft Eyes), hands	Face and neck primarily
Results Timeline	Gradual (visible after 2–3 sessions)	Fast (noticeable glow after first session)
Longevity	6–9 months	4–6 months
Technique	Varies by product and indication (e.g. Nucleofill injection points)	BAP technique (standardized)

Which Treatment Should You Choose?

Deciding between Nucleofill or Profhilo isn’t always straightforward; both offer unique advantages, but the best choice depends on your client’s skin type, age, concerns, and treatment goals. These two skin boosters may seem similar on the surface, but their mechanisms of action, formulations, and long-term outcomes set them apart.

Nucleofill is a powerful option for patients looking for deep, regenerative skin therapy. Thanks to its polynucleotide-based structure, it goes beyond surface hydration. It actively stimulates fibroblast cells, encourages collagen and elastin synthesis, and improves microcirculation and detoxification. This makes it ideal for older clients, those with visible signs of aging, or patients with environmentally damaged, inflamed, or dehydrated skin. It’s also a go-to for targeting more delicate areas like under the eyes (with Nucleofill Soft Eyes) or for firming the neck and décolletage using Nucleofill Strong.

On the other hand, Profhilo is a brilliant choice for quick hydration, firmness, and glow, especially in younger clients or those seeking subtle but noticeable skin improvements without downtime. Because it contains stabilized hyaluronic acid with a unique formulation that spreads evenly, it delivers fast results with minimal injections. If your client is prepping for an event, just starting to notice skin laxity, or prefers low-commitment, hydration-first treatments, Profhilo is an excellent entry-level skin booster.

Choose Nucleofill if:

• Your client has damaged, inflamed, or aging skin
• They want long-term skin quality improvement rather than instant plumping
• You’re targeting under-eye hollowness, neck laxity, or deep dehydration
• They’ve already tried HA boosters and want something regenerative

To offer your clients the latest in non-surgical rejuvenation, you can explore options and choose whether you want to buy Nucleofill products or you’d rather buy Polynucleotides online from Best Buy Fillers shop.

Choose Profhilo if:

• Your client needs a quick hydration boost
• They’re just starting with injectables
• Their skin lacks elasticity but is not deeply damaged
• They want a visible glow in a short time frame

Combining the Two: Can You Use Nucleofill and Profhilo Together?

Yes, many advanced practitioners now combine these treatments for synergistic effects. For example:

• Profhilo to rapidly hydrate and lift the skin
• Nucleofill to strengthen skin structure and fight oxidative damage

These protocols may alternate sessions every few weeks or use the products in different areas depending on the indication.

Real-World Feedback: What Do Patients and Practitioners Say?

The increasing demand for “regenerative injectables” has led to a rise in positive Nucleofill reviews, especially for hard-to-treat areas like the neck and under-eyes.

Popular search trends like “Nucleofill near me” reflect growing awareness, and a growing number of positive Nucleofill reviews indicate that the patients are very satisfied with the results. We wrote in detail about Nucleofill skin booster on our blog for your convenience.

Clinical evidence supports the use of Nucleofill skin booster in:

• Improving skin density
• Reducing puffiness and pigmentation (especially with Nucleofill Soft Eyes)
• Supporting filler-free rejuvenation protocols

Meanwhile, Profhilo remains a gold standard for hydration-first treatments.

Frequently Asked Questions

Can you use Nucleofill and Profhilo together?

Yes. Many medical professionals use them in alternating sessions or in complementary areas for enhanced skin texture and regeneration.

What is stronger than Profhilo?

In terms of skin regeneration, Nucleofill is considered more “active” because it stimulates fibroblasts and detoxifies the skin. While Profhilo hydrates quickly, Nucleofill Strong delivers deeper, longer-lasting structural improvements.

What are the benefits of Nucleofill?

Nucleofill enhances skin firmness, hydration, elasticity, and cell regeneration. It also reduces oxidative stress and supports long-term tissue health.

What are the disadvantages of Profhilo?

While safe and effective, Profhilo:

• May offer only short-term hydration
• Is less effective for severely aged or inflamed skin
• Requires maintenance every 4–6 months

Is Nucleofill a strong filler?

No, Nucleofill is not a filler. Even Nucleofill Strong is a biostimulator, not a volumizer. It does not add bulk but enhances the skin’s natural structure and function.

Conclusion

If you’re debating between Nucleofill or Profhilo, the answer isn’t always one or the other. Instead, the best results often come from understanding their unique strengths and tailoring protocols to patient needs.

• For hydration and glow, go with Profhilo.
• For true regeneration, resilience, and collagen repair, choose Nucleofill.

For optimal results? Combine them.

Sources

1. Lee KWA, Chan KWL, Lee A, et al. “Polynucleotides in Aesthetic Medicine: A Review of Current Practices and Perceived Effectiveness.” Int J Mol Sci. 2024;25(15):8224. Published 2024 Jul 27. doi:10.3390/ijms25158224 https://pmc.ncbi.nlm.nih.gov/articles/PMC11311621/
2. Humzah D, Molina B, Salti G, Cigni C, Bellia G, Grimolizzi F. Intradermal Injection of Hybrid Complexes of High- and Low-Molecular-Weight Hyaluronan: Where Do We Stand and Where Are We Headed in Regenerative Medicine?. Int J Mol Sci. 2024;25(6):3216. Published 2024 Mar 12. doi:10.3390/ijms25063216
3. Borrelli, A., et al. Polynucleotides in Aesthetic Medicine: New Regenerative Options. J Cosmet Dermatol. 2021; 20(9):2701–2708. https://pmc.ncbi.nlm.nih.gov/articles/PMC11311621/